Alcian Blue (Ph 2.5) stains acid mucosubstances and acetic mucins. Strongly acidic mucosubstances will be stained blue, nuclei will be stained pink to red, and cytoplasm will be stained pale pink. It is one of the most widely used cationic dyes for both light and electron microscopy. Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods
Alcian Blue (Ph 2.5) stains acid mucosubstances and acetic mucins. Strongly acidic mucosubstances will be stained blue, nuclei will be stained pink to red, and cytoplasm will be stained pale pink. It is one of the most widely used cationic dyes for both light and electron microscopy. Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods