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UNIspread Positively Charged Slides-Hydrophilic
Tissue loss during immunohistochemical staining procedures may occur due to weak interaction of a tissue sample with glass surfaces. Histological methods for preparing tissue sections for microscopic examination commonly use glass slides with a modified surface chemistry enhance tissue adhesion. Such surface modifications typically include imparting a net positive electrical charge to the slide by treatment with agents such as aminosilane, polylysine, or other proprietary chemistries. However, even with such surface modifications, a proportion of tissues subjected to HIER are lifted, folded, damaged, or completely detached from slides after thermal treatment. Although partial lifting or folding of tissue sections may not significantly impact morphological assessment, the IHC results may be severely compromised, particularly for small samples such as needle biopsies. At PathnSitu we compared the ability of hydrophobic and hydrophilic slides in two functional areas common in IHC:tissue retention and reagent dispersal. Slides that display superior tissue adhesion ability and superior reagent dispersement could be of great benefit to laboratories seeking to minimize incidences of tissue samples loss and, as an added benefit, could reduce laboratory reagent costs—since greater reagent dispersal can lead to less reagent use.
Tissue loss during immunohistochemical staining procedures may occur due to weak interaction of a tissue sample with glass surfaces. Histological methods for preparing tissue sections for microscopic examination commonly use glass slides with a modified surface chemistry enhance tissue adhesion. Such surface modifications typically include imparting a net positive electrical charge to the slide by treatment with agents such as aminosilane, polylysine, or other proprietary chemistries. However, even with such surface modifications, a proportion of tissues subjected to HIER are lifted, folded, damaged, or completely detached from slides after thermal treatment. Although partial lifting or folding of tissue sections may not significantly impact morphological assessment, the IHC results may be severely compromised, particularly for small samples such as needle biopsies. At PathnSitu we compared the ability of hydrophobic and hydrophilic slides in two functional areas common in IHC:tissue retention and reagent dispersal. Slides that display superior tissue adhesion ability and superior reagent dispersement could be of great benefit to laboratories seeking to minimize incidences of tissue samples loss and, as an added benefit, could reduce laboratory reagent costs—since greater reagent dispersal can lead to less reagent use.
