PAS-Diastase stain refers to the Pas stain used in combination with diastase enzyme to differentiate glycogen from PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. The PAS with Diastase staining procedure can also be used to differentiate glycogen granules from other granules in various tumor types. Mucin can be specifically identified in certain tissue samples using the PAS staining procedure only if the glycogen (which is also PAS-positive) is digested with diastase and washed out. In cirrhosis, α1-AT globules characteristically occur at the periphery of the nodules in multiple sizes within the hepatocyte gives a dark, reddish-purple when stained with PAS-diastase as glycogen is digested by diastase.
PAS-Diastase stain refers to the Pas stain used in combination with diastase enzyme to differentiate glycogen from PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. The PAS with Diastase staining procedure can also be used to differentiate glycogen granules from other granules in various tumor types. Mucin can be specifically identified in certain tissue samples using the PAS staining procedure only if the glycogen (which is also PAS-positive) is digested with diastase and washed out. In cirrhosis, α1-AT globules characteristically occur at the periphery of the nodules in multiple sizes within the hepatocyte gives a dark, reddish-purple when stained with PAS-diastase as glycogen is digested by diastase.
