Mayer’s Hematoxylin
This histological staining reagent is suitable for visualization of nuclei in tissue sections and cell preparations. This reagent does not contain alcohol and is suitable for use with all chromogens commonly used in immunohistochemical (IHC) applications. It may also be used for routine Hematoxylin and Eosin staining. Hematoxylin solutions contain hematein and a metal mordant. The mordant provides the stain color. In the case of Mayer’s Hematoxylin, the hematoxylin contains aluminum, giving a blue color. Mayer’s Hematoxylin is a progressive stain which does not require acid wash after the hematoxylin step. For histochemical purposes, the progressive staining is commonly used. Slides are left in the hematoxylin solution only long enough to stain the nuclei. After staining, tissues are blued in tap water or ammonia solution that has been slightly alkalinized.
This histological staining reagent is suitable for visualization of nuclei in tissue sections and cell preparations. This reagent does not contain alcohol and is suitable for use with all chromogens commonly used in immunohistochemical (IHC) applications. It may also be used for routine Hematoxylin and Eosin staining. Hematoxylin solutions contain hematein and a metal mordant. The mordant provides the stain color. In the case of Mayer’s Hematoxylin, the hematoxylin contains aluminum, giving a blue color. Mayer’s Hematoxylin is a progressive stain which does not require acid wash after the hematoxylin step. For histochemical purposes, the progressive staining is commonly used. Slides are left in the hematoxylin solution only long enough to stain the nuclei. After staining, tissues are blued in tap water or ammonia solution that has been slightly alkalinized.
