Masson's Trichrome
Masson's trichrome staining is widely used to study muscular pathologies (muscular dystrophy), cardiac pathologies (infarct), hepatic pathologies (cirrhosis) or kidney pathologies (glomerular fibrosis). It can also be used to detect and analyze tumors on hepatic and kidney biopsies. In Trichome staining,three dyes are employed to selectively stain muscle, collagen fibers, fibrin, and erythrocytes.The less porous tissues are colored by the smallest dye molecule; whenever a dye of large moleculer size is able to penetrate, it will always do so at the expense of the smaller molecule.The tissue is stained first with the acid dye which binds with the acidophilic tissue components.Then when treated with the phospho acids, the less permeable components. Then when treated with the red is pulled out of the collagen. At the same time causing a link with the collagen to bind with the aniline blue.
Masson's trichrome staining is widely used to study muscular pathologies (muscular dystrophy), cardiac pathologies (infarct), hepatic pathologies (cirrhosis) or kidney pathologies (glomerular fibrosis). It can also be used to detect and analyze tumors on hepatic and kidney biopsies. In Trichome staining,three dyes are employed to selectively stain muscle, collagen fibers, fibrin, and erythrocytes.The less porous tissues are colored by the smallest dye molecule; whenever a dye of large moleculer size is able to penetrate, it will always do so at the expense of the smaller molecule.The tissue is stained first with the acid dye which binds with the acidophilic tissue components.Then when treated with the phospho acids, the less permeable components. Then when treated with the red is pulled out of the collagen. At the same time causing a link with the collagen to bind with the aniline blue.
